Isolation, identification and characterization of a novel elastase from Chryseobacterium indologenes

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Identification and characterization of a new metallo-beta-lactamase, IND-5, from a clinical isolate of Chryseobacterium indologenes.

A new natural IND-type metallo-beta-lactamase variant, IND-5, was identified in a clinical isolate of Chryseobacterium indologenes. IND-5 shared 92.8% and 92.4% amino acid homology with IND-1 and IND-3, respectively. Purified enzyme (pI = 8.8, M(r) = 25,000) was able to hydrolyze penicillins, some narrow- and expanded-spectrum cephalosporins, and carbapenems but not monobactams.

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Identification and Characterization of a New Metallo- -Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes

Identification and Characterization of a New Metallo-Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes Mariagrazia Perilli, Bibiana Caporale, Giuseppe Celenza, Cristina Pellegrini, Jean Denis Docquier, Marilina Mezzatesta, Gian Maria Rossolini, Stefania Stefani, and Gianfranco Amicosante* Dipartimento di Scienze e Tecnologie Biomediche, Università di L’Aquila, I-67100 L’...

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Chryseobacterium indologenes bacteraemia in a diabetic child.

Chryseobacterium indologenes is a non-fermentative Gram-negative bacillus that is a rare pathogen in humans. Its occurrence in diabetic children has not been previously reported. In this report, a case is described of C. indologenes bacteraemia possibly associated with the use of a peripheral venous catheter. A 2-year-old boy with type I diabetes mellitus was admitted due to a coma caused by ce...

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Characterization of CIA-1, an Ambler class A extended-spectrum β-lactamase from Chryseobacterium indologenes.

An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition...

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Identification and virulence of Chryseobacterium indologenes isolated from diseased yellow perch (Perca flavescens).

AIM To identify pathogen of diseased yellow perch and determine their virulence. METHODS AND RESULTS Fifteen Gram-negative bacterial isolates were recovered from the skin lesions of diseased yellow perch (Perca flavescens). Based on API 20NE test, ten isolates were found to share 67.2-99.9% homologies with Chryseobactertium indologenes. Based on fatty acid methyl ester analysis, 13 isolates w...

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ژورنال

عنوان ژورنال: Applied Biological Chemistry

سال: 2018

ISSN: 2468-0834,2468-0842

DOI: 10.1007/s13765-018-0364-6